LOCUS       sequence-69797-e        3413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3413)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET Mocr TEV expression vector with BioBrick
            p...
FEATURES             Location/Qualifiers
     source          1..3413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      48..94
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      154..181
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        207..225
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             242..264
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             652..672
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      714..760
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1927..1966
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1997..2585
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2486..2505
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2739..2756
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2771..2910
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2836..2855)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2996..3018
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3012..3203)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaatgcgg ccgcttaatt aaggcatcaa ataaaacgaa
       61 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg agtccttcca
      121 gtctgggcga aagcgcggtc gatcaaatta agcagaaggc catcctgacg gatggccttt
      181 ttgcgtttct atcgatcccg cgaaattaat acgactcact ataggcccct ctagaaataa
      241 ttttgtttaa ctttaagaag gagatatacc atgggttctt ctggttcttc tatgagcaac
      301 atgacataca ataatgtttt cgaccacgcg tacgaaatgc ttaaggagaa catccgttat
      361 gatgacatac gggatacaga tgaccttcac gacgcaattc atatggccgc cgataatgcc
      421 gtccctcact attatgccga tattcggtca gttatggcat ctgaaggtat tgatctggaa
      481 tttgaggata gtggcctcat gccagatacg aaggacgaca tcagaattct gcaagcgcgg
      541 atctacgaac aacttaccat cgatttatgg gaagatgcgg aagatctcct gaatgaatac
      601 ctggaagaag tggaggagta tgaggaagat gaagagggga cggggatcga ggaaaacctg
      661 tacttccaat ccaatattgg aagtggataa ctggctgctg ccaccgctga gcatagcata
      721 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      781 aagtcccgaa gtagctgtca gactgctggc gaccgccgca actagtggcg tcatgcgtgc
      841 cgtatggtct ttcgcctgta gacgccctac aagccgtact aaagtggaaa gacccgcaaa
      901 aggtatcgcg atcgccctgc agggaactat atccgactag tagcccgcct aatgagcggg
      961 ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1021 atgagacaat aaccctgata aatgcttcga aaaaggaaga gtatgagtat tcaacatttc
     1081 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     1141 acgctcgtga aagtaaaaga cgcagaggac caattggggg cacgagtggg atacatagaa
     1201 ctggacttga atagcggtaa aatccttgag agttttcgcc ctgaagagcg ttttccaatg
     1261 atgagcactt tcaaagttct gctatgtgga gcagtattat cccgtgtaga tgcggggcaa
     1321 gagcaactcg gacgacgaat acactattcg cagaatgact tggttgaata ctccccagtg
     1381 acagaaaagc accttacgga cggaatgacg gtaagagaat tatgtagtgc cgccataacg
     1441 atgagtgata acactgcggc gaacttactt ctgacaacca tcggtggacc gaaggaatta
     1501 accgcttttt tgcacaatat gggagaccat gtaactcgcc ttgaccgttg ggaaccagaa
     1561 ctgaatgaag ccataccaaa cgacgagcga gacaccacaa tgcctgcggc aatggcaaca
     1621 acattacgca aactattaac tggcgaacta cttactctgg cttcacggca acaattaata
     1681 gactggcttg aagcggataa agttgcagga ccactactgc gttcggcact tcctgctggc
     1741 tggtttattg ctgataaatc tggggcagga gagcgtggtt cacggggtat cattgccgca
     1801 cttggaccag atggtaagcc ttcccgtatc gtagttatct acacgacggg tagtcaggca
     1861 actatggacg aacgaaatag acagattgct gaaatagggg cttcactgat taagcattgg
     1921 taaaccgata caattaaagg ctccttttgg agcctttttt tttggacgga ccggtagaaa
     1981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     2161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     2221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     2281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     2341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     2401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     2461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     2521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     2581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     2641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     2701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     2761 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2821 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     2881 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     2941 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3001 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     3061 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     3121 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     3181 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     3241 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     3301 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     3361 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gtt
//
