LOCUS       sequence-69820-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD18 
            efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB3994
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(346..351,352..368,369..375)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
       61 catgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt tactttgcag
      121 ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtcga attcaaaaga
      181 tcttaagtaa gtaagagtat acgtatatcg gctaataacg tattaaggcg cttcggcgcc
      241 tttttttatg ggggtatttt catcccaatc cacacgtcca acgcacagca aacaccacgt
      301 cgaccctatc agctgcgtgc tttctatgag tcgttgctgc ataacttgac aattaatcat
      361 ccggctcgta taatgtgtgg agggcccaag ttcacttaaa aaggagatca acaatgaaag
      421 caattttcgt actgaaacat cttaatcatg cgacggagcg tttctaatgg cgagtagcga
      481 agacgttatc aaagagttca tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca
      541 cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa
      601 actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt
      661 ccagtacggt tccaaagctt acgttaaaca cccggctgac atcccggact acctgaaact
      721 gtccttcccg gaaggtttca aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt
      781 taccgttacc caggactcct ccctgcaaga cggtgagttc atctacaaag ttaaactgcg
      841 tggtaccaac ttcccgtccg acggtccggt tatgcagaaa aaaaccatgg gttgggaagc
      901 ttccaccgaa cgtatgtacc cggaaga
//
