LOCUS       sequence-69887-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a Gateway destination vector for secreted expression in 
            Drosophila cell culture by CuSO4 induction. Inserts will be 
            pentamerized and tagged with Alkaline phosphatase, FLAG and His 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Ozkan E, Carrillo RA, Eastman CL, Weiszmann R, Waghray D, Johnson 
            KG, Zinn K, Celniker SE, Garcia KC
  TITLE     An Extracellular Interactome of Immunoglobulin and LRR Proteins 
            Reveals Receptor-Ligand Networks.
  JOURNAL   Cell. 2013 Jul 3;154(1):228-39. doi: 10.1016/j.cell.2013.06.006.
  PUBMED    23827685
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "228-39"
COMMENT     Sequence Label: pECIA14
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(109..233)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             259..282
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg
       61 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg
      121 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      181 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggttgatatc
      241 cagcacagtg gcggccgcct ggaggtgctg ttccagggac caagcggcaa tcccggcggc
      301 gatctggccc cccagatgct gcgcgagctg caggagacca atgccgccct gcaggacgtg
      361 cgcgagctgc tgcgccaccg cgtgaaggag atcaccttcc tgaagaacac cgtgatggag
      421 tgcgacgcct gcggcatgca gccctctaga ggctccggct ccatcatccc agttgaggag
      481 gagaacccgg acttctggaa ccgcgaggca gccgaggccc tgggtgccgc caagaagctg
      541 cagcctgcac agacagccgc caagaacctc atcatcttcc tgggcgatgg gatgggggtg
      601 tctacggtga cagctgccag gatcctaaaa gggcagaaga aggacaaact ggggcctgag
      661 atacccctgg ccatggaccg cttcccatat gtggctctgt ccaagacata caatgtagac
      721 aaacatgtgc cagacagtgg agccacagcc acggcctacc tgtgcggggt caagggcaac
      781 ttccagacca ttggcttgag tgcagccgcc cgctttaacc agtgcaacac gacacgc
//
