LOCUS       sequence-69888-e         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is a Gateway destination vector for secreted expression in 
            Drosophila cell culture by CuSO4 induction. Inserts will be 
            pentamerized and tagged with Alkaline phosphatase, FLAG and His 
            tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Ozkan E, Carrillo RA, Eastman CL, Weiszmann R, Waghray D, Johnson 
            KG, Zinn K, Celniker SE, Garcia KC
  TITLE     An Extracellular Interactome of Immunoglobulin and LRR Proteins 
            Reveals Receptor-Ligand Networks.
  JOURNAL   Cell. 2013 Jul 3;154(1):228-39. doi: 10.1016/j.cell.2013.06.006.
  PUBMED    23827685
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-3- 3";
            volume: "154"; issue: "1"; pages: "228-39"
COMMENT     Sequence Label: pECIA14
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..41
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(64..81)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(97..120)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ggctgatcag cgagctcagg ccttagaagg cacagtcgag gctgatcagc gggtttaaac
       61 tcaatggtga tggtgatgat gaccggtgcc gctggcctta tcatcatcat ccttgtaatc
      121 gttatcgccg gaggtgcttc ctctagaacc cgggtgcgcg gcgtcggtgg tgccggcggg
      181 gggcgccagg tcgcaggcgg tgtagggctc caggcaggcg gcgaaggcca tgacgtgcgc
      241 tatgaaggtc tgctcctgca cgccgtgaac caggtgcgcc tgcgggccgc gcgcgaacac
      301 cgccacgtcc tcgcctgcgt gggtctcttc gtccaggggc actgctgact gctgccgata
      361 ctcggggctc ccgctctcgc tctcggtaac atccggccgg gcgccgtcct tgagcacata
      421 gcctggaccg tttccgtata ggaggaccgt gtaggccttc ctgtcccggg ccttgccagg
      481 ggccagcccg aagatggagc tccctcgcag ggggtagcct ccgaaggaga agacgtggga
      541 gtggtcggca gtgacgaggc tcagcgtgtc ctcctcgctg gtgagctggc ccgccctctc
      601 aatggcgtcg tcgaacatga tcgtctcagt cagtgcccgg taagccctgc tttcatgatg
      661 accatggtcg atgcgaccac cctccacgaa gaggaagaag ccgcgggggt tcctgctcag
      721 caggcgcagg gcagcctctg tcatctccat cagggagggg tccagtgtgg agtctcggtg
      781 gatctcgtat ttcatgtctc caggctcaaa ga
//
