LOCUS       sequence-69892-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Chicka MC, Strehler EE
  TITLE     Alternative splicing of the first intracellular loop of plasma 
            membrane Ca2+-ATPase isoform 2 alters its membrane targeting.
  JOURNAL   J Biol Chem. 2003 May 16;278(20):18464-70. Epub 2003 Mar 6.
  PUBMED    12624087
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2003-05-16"; volume: "278(20)"; pages: "18464-70. 
            Epub 2003 Mar 6"
COMMENT     Sequence Label: EGFP-hPMCA2z/b
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..44
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             63..80
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      132..141
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(639..650)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agtccggact cagatcctac ccctatgatg tgcctgacta tgcccaccac caccaccacc
       61 acctggtgcc caggggcagc agatctcgag ctcaagcttc gaattctgca gtcgacggta
      121 ccgcggtggc ggccgccatg ggtgacatga ccaacagcga cttttactcc aaaaaccaaa
      181 gaaatgagtc gagccatggg ggcgagttcg ggtgcacaat ggaggagctc cgctccctca
      241 tggagctgcg gggcactgag gctgtggtca agatcaagga gacttatggg gacaccgaag
      301 ccatctgccg gcgcctcaaa acctcacctg ttgaaggttt gccggacacc gctccagacc
      361 tggaaaagag aaagcaaatt tttgggcaaa actttatacc tccaaagaag ccaaaaacct
      421 tcctgcagct cgtgtgggag gcgctgcagg acgtgacgct catcatcctg gagattgccg
      481 ccatcatctc cctggggctg tccttctacc acccgcccgg cgagggcaac gaaggatgtg
      541 cgacggccca gggtggggca gaggatgaag gagaggcaga ggcaggttgg atcgaggggg
      601 ccgccattct cctctcagtt atctgtgtgg tcctggtcac ggccttcaat gactggagca
      661 aagagaaaca gttccggggc ctgcagagcc gcatcgagca ggaacagaaa tttaccgtag
      721 tccgggctgg ccaggtggtg cagatccctg tggctgagat cgtggttggg gacat
//
