LOCUS       sequence-69906-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant TVA-mCherry fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-TCB
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      452..461
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(468..501)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(572..605)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(682..702)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     gap             947..958
                     /estimated_length=12
ORIGIN
        1 tnncncncct tgctcncccc accccccttg gatgcgcttt caagactgaa gatgtcagac
       61 ctgctttttg ctttggactt cccccatgca gcgataccac ccacagctac caggcagcac
      121 aggagcactg cagtgatcag catccacatg cggccgtgat tcctggctgg cagagcacgt
      181 ccaggagcag gggcagtggg agcctctgtg ccgttgtccg tgggcaccgc ggggatcgcg
      241 ctggtcccgc agccccactc gtcccgcccg tcgtcgcagt cggggtgtcc gtcgcacagc
      301 cagtcctgcg ggtaacactc cccgtgggca ccgggcggct ccgagcagcg gaactgaccg
      361 ggggggcaac gggacaaaga accgttaccg gacccgttac cggtcacgtt accgggcagc
      421 agcagcagca gcagcgcggg cagcagccgc gccatggtgg cgcgcctata acttcgtata
      481 ggatacttta tacgaagtta tcattgggat tcttcctatt ttgatccaag catcaccatc
      541 gaccctctag tccagatctc accatcgacc cataacttcg tatagcatac attatacgaa
      601 gttataagct tatatgcggc cgatcgataa tcaacctctg gattacaaaa tttgtgaaag
      661 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
      721 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
      781 ctggttgctg tctctttatg aggagttgtg gcccgttgtc agcaacgtgg ngtgngtgca
      841 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
      901 ccggganttt cgctttcccc ctccctattg ccacggcnga nncatcnnnn nnnnnnnngc
      961 ngctgganag ggctcgnctn nggnacngan atnc
//
