LOCUS       sequence-69908-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependant TVA-mCherry fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Miyamichi K, Shlomai-Fuchs Y, Shu M, Weissbourd BC, Luo L, Mizrahi A
  TITLE     Dissecting local circuits: parvalbumin interneurons underlie broad 
            feedback control of olfactory bulb output.
  JOURNAL   Neuron. 2013 Dec 4;80(5):1232-45. doi: 10.1016/j.neuron.2013.08.027.
            Epub 2013 Nov 14.
  PUBMED    24239125
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.08.027"; journalName: "Neuron"; date: 
            "2013-12-4- 4"; volume: "80"; issue: "5"; pages: "1232-45"
COMMENT     Sequence Label: CAG-Flex-TCB
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..117
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    188..221
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      228..237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     gap             708..720
                     /estimated_length=13
ORIGIN
        1 ggcacntang nttannatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt
       61 atcgatcggc cgcatataag cttataactt cgtataatgt atgctatacg aagttatggg
      121 tcgatggtga gatctggact agagggtcga tggtgatgct tggatcaaaa taggaagaat
      181 cccaatgata acttcgtata aagtatccta tacgaagtta taggcgcgcc accatggcgc
      241 ggctgctgcc cgcgctgctg ctgctgctgc tgcccggtaa cgtgaccggt aacgggtccg
      301 gtaacggttc tttgtcccgt tgcccccccg gtcagttccg ctgctcggag ccgcccggtg
      361 cccacgggga gtgttacccg caggactggc tgtgcgacgg acaccccgac tgcgacgacg
      421 ggcgggacga gtggggctgc gggaccagcg cgatccccgc ggtgcccacg gacaacggca
      481 cagaggctcc cactgcccct gctcctggac gtgctctgcc agccaggaat cacggccgca
      541 tgtggatgct gatcactgca gtgctcctgt gctgcctggt agctgtgggt ggtatcgctg
      601 catgggggaa gtccaaagca aaaagcaggt ctgacatctt cagtcttgaa agcgcatcca
      661 aggggggtgg ggtgagcaag ggcgaggagg ataacatggc catcatcnnn nnnnnnnnnn
      721 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      781 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaangtga ccaaggnggc
      841 cccctgcccn tcgcctggga catcctgtcc cctcanttca tgtacggctc caaggcnnac
      901 ntgaagcacc cgccgacntc cccgactact tgaagctgtc cttccccgag ggctcagtgg
      961 gagcgcnnga tgactnnaga ngnnnnnnnn acgngacca
//
