LOCUS       sequence-69920-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD23 
            efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB3999
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(327..332,333..349,350..356)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 caatcatgcg aaacgatcct catcctgtct cttgatcaga tcatgatccc ctgcgccatc
       61 agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca accttaccag
      121 agggcgcccc agctggcaat tccgacgtcg aattcaaaag atcttaagta agtaagagta
      181 tacgtatatc ggctaataac gtattaaggc gcttcggcgc ctttttttat gggggtattt
      241 tcatcccaat ccacacgtcc aacgcacagc aaacaccacg tcgaccctat cagctgcgtg
      301 ctttctatga gtcgttgctg cataacttga caattaatca tccggctcgt ataatgtgtg
      361 gagggcccaa gttcacttaa aaaggagatc aacaatgaaa gcaattttcg tactgaaaca
      421 tcttaatcat gcgaaggaca gtttctaatg gcgagtagcg aagacgttat caaagagttc
      481 atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga aatcgaaggt
      541 gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt taccaaaggt
      601 ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg ttccaaagct
      661 tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc ggaaggtttc
      721 aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac ccaggactcc
      781 tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa cttcccgtcc
      841 ga
//
