LOCUS       sequence-69957-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring rnpB T1 termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB815
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             8..19
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(261..938)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
ORIGIN
        1 tggtaccttt ctcctcttta atgaattgat taaccggaag aaggtcaatc ataaaggcca
       61 ctcttgcgaa tgaccttgag tttgtccctc tatagtgagt ccgccgacag tcattcatct
      121 ttctgcccct ccaaaagcaa aaacccgccg aagcgggttt ttacgtaaat caggtgaaac
      181 tgaccgaatc acataagtaa agtgataatc ataaaggcca ctcgctagga gcgaccttga
      241 gtctatcact cagcagatta ttaagcaccg gtggagtgac gaccttcagc acgttcgtac
      301 tgttcaacga tggtgtagtc ttcgttgtgg gaggtgatgt ccagtttgat gtcggttttg
      361 taagcacccg gcagctgaac cggtttttta gccatgtagg tggttttaac ttcagcgtcg
      421 tagtgaccac cgtctttcag tttcagacgc attttgattt cacctttcag agcaccgtct
      481 tccgggtaca tacgttcggt ggaagcttcc caacccatgg tttttttctg cataaccgga
      541 ccgtcggacg ggaagttggt accacgcagt ttaactttgt agatgaactc accgtcttgc
      601 agggaggagt cctgggtaac ggtaacaaca ccaccgtctt cgaagttcat aacacgttcc
      661 catttgaaac cttccgggaa ggacagtttc aggtagtccg ggatgtcagc cgggtgttta
      721 acgtaagctt tggaaccgta ctggaactgc ggggacagga tgtcccaagc gaacggcagc
      781 ggaccacctt tggtaacttt cagtttagcg gtctgggtac cttcgtacgg acgaccttca
      841 ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg aactttgaaa
      901 cgcatgaact ctttgataac gtcttcgcta ctcgccatgg
//
