LOCUS       sequence-69964-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a template from which gRNAs can be made via InFusion 
            cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For 
            use in plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Jacobs TB, LaFayette PR, Schmitz RJ, Parrott WA
  TITLE     Targeted genome modifications in soybean with CRISPR/Cas9.
  JOURNAL   BMC Biotechnol. 2015 Mar 12;15:16. doi: 10.1186/s12896-015-0131-2.
  PUBMED    25879861
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2015-03-12"; volume: "15:16. doi"; pages: " 
            10.1186/s12896-015-0131-2"
COMMENT     Sequence Label: pUC gRNA Shuttle
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        403..478
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(511..528)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(511..527)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(520..542)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(736..755)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cttattaccc tgttatccct agatatcgat tttggctacc ttaaggtagc atgcctatct
       61 tatatgatca atgaggcatt taattgggtg catatgatgg tgaaaaaagg tgcagctcct
      121 ggcttgggaa tgatgactca tgtggaattt ggtcttaaat ttatcacatc cttttgggat
      181 gtgatgattg tatcacttgt tcattttgca aagacaaggt gcactgctac aaactttggt
      241 ttaatctgaa ataaaacaaa actcactgag aggaagatgc atcccagtag gtgaaagtcg
      301 agaaggattt gcatgttact attacacttg ctttttagtc ccacatcgtc tgaaacataa
      361 aatatttcag cgtttaaata cttcaagcga accagtaggc ttgttttaga gctagaaata
      421 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      481 tttttgctac cttaagagag tcatgaattc actggccgtc gttttacaac gtcgtgactg
      541 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      601 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      661 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
      721 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
      781 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
      841 agctgtgacc g
//
