LOCUS       sequence-69968-e         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Chicka MC, Strehler EE
  TITLE     Alternative splicing of the first intracellular loop of plasma 
            membrane Ca2+-ATPase isoform 2 alters its membrane targeting.
  JOURNAL   J Biol Chem. 2003 May 16;278(20):18464-70. Epub 2003 Mar 6.
  PUBMED    12624087
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2003-05-16"; volume: "278(20)"; pages: "18464-70. 
            Epub 2003 Mar 6"
COMMENT     Sequence Label: EGFP-hPMCA2w/b
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..42
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      130..139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(637..648)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tccggactca gatcctaccc ctatgatgtg cctgactatg cccaccacca ccaccaccac
       61 ctggtgccca ggggcagcag atctcgagct caagcttcga attctgcagt cgacggtacc
      121 gcggtggcgg ccgccatggg tgacatgacc aacagcgact tttactccaa aaaccaaaga
      181 aatgagtcga gccatggggg cgagttcggg tgcacaatgg aggagctccg ctccctcatg
      241 gagctgcggg gcactgaggc tgtggtcaag atcaaggaga cttatgggga caccgaagcc
      301 atctgccggc gcctcaaaac ctcacctgtt gaaggtttgc cggacaccgc tccagacctg
      361 gaaaagagaa agcaaatttt tgggcaaaac tttatacctc caaagaagcc aaaaaccttc
      421 ctgcagctcg tgtgggaggc gctgcaggac gtgacgctca tcatcctgga gattgccgcc
      481 atcatctccc tggggctgtc cttctaccac ccgcccggcg agggcaacga aggatgtgcg
      541 acggcccagg gtggggcaga ggatgaagga gaggcagagg caggttggat cgagggggcc
      601 gccattctcc tctcagttat ctgtgtggtc ctggtcacgg ccttcaatga ctggagcaaa
      661 gagaaacagt tccggggcct gcagagccgc atcgagcagg aacagaaatt tacc
//
