LOCUS       sequence-69971-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Chicka MC, Strehler EE
  TITLE     Alternative splicing of the first intracellular loop of plasma 
            membrane Ca2+-ATPase isoform 2 alters its membrane targeting.
  JOURNAL   J Biol Chem. 2003 May 16;278(20):18464-70. Epub 2003 Mar 6.
  PUBMED    12624087
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2003-05-16"; volume: "278(20)"; pages: "18464-70. 
            Epub 2003 Mar 6"
COMMENT     Sequence Label: EGFP-hPCM2w/b delta6
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..33
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..69
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      121..130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(628..639)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agatcctacc cctatgatgt gcctgactat gcccaccacc accaccacca cctggtgccc
       61 aggggcagca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcggtggcg
      121 gccgccatgg gtgacatgac caacagcgac ttttactcca aaaaccaaag aaatgagtcg
      181 agccatgggg gcgagttcgg gtgcacaatg gaggagctcc gctccctcat ggagctgcgg
      241 ggcactgagg ctgtggtcaa gatcaaggag acttatgggg acaccgaagc catctgccgg
      301 cgcctcaaaa cctcacctgt tgaaggtttg ccggacaccg ctccagacct ggaaaagaga
      361 aagcaaattt ttgggcaaaa ctttatacct ccaaagaagc caaaaacctt cctgcagctc
      421 gtgtgggagg cgctgcagga cgtgacgctc atcatcctgg agattgccgc catcatctcc
      481 ctggggctgt ccttctacca cccgcccggc gagggcaacg aaggatgtgc gacggcccag
      541 ggtggggcag aggatgaagg agaggcagag gcaggttgga tcgagggggc cgccattctc
      601 ctctcagtta tctgtgtggt cctggtcacg gccttcaatg actggagcaa agagaaacag
      661 ttccggggcc tgcagagccg catcg
//
