LOCUS       sequence-70024-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET reporter for Ca2+ inside primary cilia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Su S, Phua SC, Derose R, Chiba S, Narita K, Kalugin PN, Katada T, 
            Kontani K, Takeda S, Inoue T
  TITLE     Genetically encoded calcium indicator illuminates calcium dynamics 
            in primary cilia.
  JOURNAL   Nat Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647.
  PUBMED    24056873
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 22. doi: 10.1038/nmeth.2647."
COMMENT     Sequence Label: TNXXL-CTS20
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(120..854)
                     /codon_start=1
                     /product="circularly permuted form of the Venus yellow 
                     fluorescent protein (Nagai et al., 2004)"
                     /label=cp173Venus
                     /note="efficient FRET acceptor for CFP"
                     /translation="MGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEELFTGVVPILVELDGDVNGHKFSVRG
                     EGEGDATNGKLTLKFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPE
                     GYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
                     VYIMADKQKNGIKANFKIRHNIE"
     primer_bind     313..332
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     572..593
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(699..720)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gnnnctagac tcgagcggcc gccactgtgc tggatatcct gcagttattc tggttttatt
       61 tttcnggttt tgcttttctt aaaccagcag caaacgagac agctcaggaa ttcatagtcc
      121 tcgatgttgt ggcggatctt gaagttggcc ttgatgccgt tcttctgctt gtcggccatg
      181 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
      241 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
      301 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
      361 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
      421 gcgaagcaca tcaggccgta gcccagggtg gtcacgaggg tgggccaggg cacgggcagc
      481 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt tggtggcatc gccctcgccc
      541 tcgccgcgca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
      601 accaccccgg tgaacagctc ctcgcccttg ctcaccatgc tgccgccggt accgcccttg
      661 tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg
      721 tgatcgcgct tctcgttggg gtctttgctc agcttggact ggtagctcag gtagtggttg
      781 tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc
      841 tgcacgccgc ccatgagctc ctgcacgccc tccatcatct tcaggaactc atcgaagtca
      901 atgcggccgt cattgttctt gtctgaatcc ttcatgaggt cttctatgtc ctcctcggtg
      961 acgtgctccc cagtggccct gaaaatctc
//
