LOCUS       sequence-70066-e        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Defective for E6AP binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Spangle J, Ghosh-Choudhury N, Munger K
  TITLE     Activation of cap dependent translation by mucosal Human 
            Papillomavirus E6 proteins is dependent on the integrity of the 
            LXXLL binding motif.
  JOURNAL   J Virol. 2012 May 2.
  PUBMED    22553330
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2012 May 2."
COMMENT     Sequence Label: pNCMV 6bE6 I127T
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             165..188
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             201..227
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(796..815)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    861..916
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(915..934)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 aatgatatac actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg
       61 ctaaccatgt tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg
      121 tgctgtctca tcattttggc aaagaattcc tcgacggatc tatggactac aaggatgacg
      181 atgacaagct cgatggagga tacccatacg atgttccaga ttacgctgat tcgactggaa
      241 tggaaagtgc aaatgcctcc acgtctgcaa cgaccataga ccagttgtgc aagacgttta
      301 atctatctat gcatacgttg caaattaatt gtgtgttttg caagaatgca ctgaccacag
      361 cagagattta ttcatatgca tataaacacc taaaggtcct gtttcgaggc ggctatccat
      421 atgcagcctg cgcgtgctgc ctagaatttc atggaaaaat aaaccaatat agacactttg
      481 attatgctgg atatgcaaca acagttgaag aagaaactaa acaagacatc ttagacgtgc
      541 taattcggtg ctacctgtgt cacaaaccgc tgtgtgaagt agaaaaggta aaacatatac
      601 taaccaaggc gcggttcaca aagctaaatt gtacgtggaa gggtcgctgc ctacactgct
      661 ggacaacatg catggaagac atgttaccct aaaagctatc ggatccgtcg aggaattcac
      721 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
      781 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
      841 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
      901 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
      961 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     1021 ttggctataa agagg
//
