LOCUS       sequence-70415-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  esaI gene downstream of esaR-a208g in pAC-EsaR-I70V (CmR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Shong J, Collins CH
  TITLE     Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent 
            Gene Expression.
  JOURNAL   ACS Synth Biol. 2013 Jul 23.
  PUBMED    23879176
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Jul 23."
COMMENT     Sequence Label: pAC-EsaR-I70V-EsaI
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    110..131
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(146..151,152..169,170..176)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    184..200
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     189..211
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             218..229
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tttctagacc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
       61 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      121 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      181 gaattgtgag cggataacaa tttcacacag gaaacataaa gaggagaaag gtacccatga
      241 tgttctcttt cttccttgaa aaccaaacaa taacggatac gcttcagact tacatacaga
      301 gaaagttatc tccgctgggt agtccggatt acgcttacac tgttgtgagc aaaaaaaatc
      361 cttcaaatgt tctgattatt tccagttatc ctgacgaatg gattaggtta taccgcgcta
      421 acaactttca gctgaccgat ccggttgttc tcacggcctt taaacgcacc tcgccgtttg
      481 cctgggatga gaatattacg ctgatgtccg acctgcggtt caccaaaatt ttctctttat
      541 ccaagcaata caacatcgtt aacggcttta cctatgtcct gcatgaccac atgaacaacc
      601 ttgctctgtt gtccgtgatc attaaaggca acgatcagac tgcgctggag caacgccttg
      661 ctgccgaaca gggcacgatg cagatgctgc tgattgattt taacgagcag atgtaccgcc
      721 tggcaggcac cgaaggtgaa cgagccccgg cgttaaatca gagcgcggac aaaacgatat
      781 tttcctcgcg tgaaaatgag gtgttgtact gggcgagtat gggcaaaacc tatgctgaga
      841 ttgccgctat
//
