LOCUS       sequence-70462-e         413 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Dolt KS, Lawrence ML, Miller-Hodges E, Slight J, Thornburn A, 
            Devenney PS, Hohenstein P
  TITLE     A universal vector for high-efficiency multi-fragment recombineering
            of BACs and knock-in constructs.
  JOURNAL   PLoS One. 2013 Apr 29;8(4):e62054. doi: 
            10.1371/journal.pone.0062054. Print 2013.
  PUBMED    23637962
REFERENCE   2  (bases 1 to 413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-04-29"; volume: "8(4):e62054. doi"; pages: " 
            10.1371/journal.pone.0062054. Print 2013"
COMMENT     Sequence Label: pENTR pA L3-L2
FEATURES             Location/Qualifiers
     source          1..413
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    83..290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(293..388)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag
       61 ctgggtaata gagcccaccg catccccagc atgcctgcta ttgtcttccc aatcctcccc
      121 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga
      181 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga
      241 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag agcaacttta
      301 ttatacaaag ttggcattat aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc
      361 actatcagtc aaaataaaat cattatttgg ggcccggccg cagggcgggc ggg
//
