LOCUS       sequence-70502-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 + sgRNA plasmid that is targeted to a genomic site near the 
            ttTi5605 Mos1 insertion allele.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Dickinson DJ, Ward JD, Reiner DJ, Goldstein B
  TITLE     Engineering the Caenorhabditis elegans genome using Cas9-triggered 
            homologous recombination.
  JOURNAL   Nat Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641.
  PUBMED    23995389
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 1. doi: 10.1038/nmeth.2641."
COMMENT     Sequence Label: pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(123..198)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(219..568)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     primer_bind     574..596
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     588..605
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     589..605
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    613..633
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
       61 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagaa atttcacaaa
      121 aagcaccgac tcggtgccac tttttcaagt tgataacgga ctagccttat tttaacttgc
      181 tatttctagc tctaaaactt acgaaacaga ctgatatcaa gacatctcgc aataggaggt
      241 gtttatatag gggcaacaca agaggcggca gacatctcgt gagtttgaca gagtagtata
      301 aagagtgtgt gtgagagggt gtaggcattt cataacatat tttttgtgga ataattttgg
      361 aaaaaaggtg tttttttaat aatagctttt ataagagttt ctcaaaaatt gcatgctttt
      421 tttgaaaagg ttgacaaatt atagtagtac taaagttgct attttcaggt ctcagcagaa
      481 aattcacaac taaaaataaa atttgcaaaa cagagtgtgc gcctttaagg tgtacagttt
      541 tttattcctt tattgctagt tttttttggt tttcccagtc acgacgttgt aaaacgacgg
      601 ccagtgaatt atcaactatg tataataaag ttgtgagact tttttcttgg cggcacaata
      661 aagtttcctc tttttgttgg gtcactcaac gaacacaata caaatatttg agcgctcgca
      721 tttttctcaa cctttcgggt ttaaagcact tcaaaatgga attgaagaga gattgtgaaa
      781 gtacatcaga cagaatcaca ttttgatagc attcacttca ctcagatgca aacttttcag
      841 tgactataaa aagtcataaa ataataacat taaaaaagca aataaattag cgagagaatt
      901 t
//
