LOCUS       sequence-70517-e        1099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MBP myocilin olfactomedin domain fusion protein with a TEV
            protease recognition sequence and the mutation E323K.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Burns JN, Turnage KC, Walker CA, Lieberman RL
  TITLE     The stability of myocilin olfactomedin domain variants provides new 
            insight into glaucoma as a protein misfolding disorder.
  JOURNAL   Biochemistry. 2011 Jul 5;50(26):5824-33. doi: 10.1021/bi200231x. 
            Epub 2011 Jun 9.
  PUBMED    21612213
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi200231x";
            journalName: "Biochemistry"; date: "2011-07-5- 5"; volume: "50"; 
            issue: "26"; pages: "5824-33"
COMMENT     Sequence Label: pmalc4x_E323K_TEV
FEATURES             Location/Qualifiers
     source          1..1099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..25
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(919..936)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(919..935)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(928..950)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttcggagaat ctttattttc agagcttgaa ggagagccca tctggctatc tcaggagtgg
       61 agagggagac accggatgtg gagaactagt ttgggtagga gagcctctca cgctgagaac
      121 agcagaaaca attactggca agtatggtgt gtggatgcga gaccccaagc ccacctaccc
      181 ctacacccag gagaccacgt ggagaatcga cacagttggc acggatgtcc gccaggtttt
      241 tgagtatgac ctcatcagcc agtttatgca gggctaccct tctaaggttc acatactgcc
      301 taggccactg aaaagcacgg gtgctgtggt gtactcgggg agcctctatt tccagggcgc
      361 tgagtccaga actgtcataa gatatgagct gaataccgag acagtgaagg ctgagaagga
      421 aatccctgga gctggctacc acggacagtt cccgtattct tggggtggct acacggacat
      481 tgacttggct gtggatgaag caggcctctg ggtcatttac agcaccgatg aggccaaagg
      541 tgccattgtc ctctccaaac tgaacccaga gaatctggaa ctcgaacaaa cctgggagac
      601 aaacatccgt aagcagtcag tcgccaatgc cttcatcatc tgtggcacct tgtacaccgt
      661 cagcagctac acctcagcag atgctaccgt caactttgct tatgacacag gcacaggtat
      721 cagcaagacc ctgaccatcc cattcaagaa ccgctataag tacagcagca tgattgacta
      781 caaccccctg gagaagaagc tctttgcctg ggacaacttg aacatggtca cttatgacat
      841 caagctttcc aagatgtgat aaggctctaa ctctcctgaa ttcggatcct ctagagtcga
      901 cctgcaggca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      961 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     1021 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcagct
     1081 tggctgtttt ggcggatga
//
