LOCUS       sequence-70531-e        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET sensor for beta2-adrenergic receptor .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Malik RU, Ritt M, Devree BT, Neubig RR, Sunahara RK, 
            Sivaramakrishnan S
  TITLE     Detection of G Protein-selective G Protein-coupled Receptor (GPCR) 
            Conformations in Live Cells.
  JOURNAL   J Biol Chem. 2013 Jun 14;288(24):17167-78. doi: 
            10.1074/jbc.M113.464065. Epub 2013 Apr 29.
  PUBMED    23629648
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M113.464065"; journalName: "J Biol Chem"; date: 
            "2013-06-14- 14"; volume: "288"; issue: "24"; pages: "17167-78"
COMMENT     Sequence Label: pCDNA_B2AR-i-pep
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             118..144
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttatgtat
      121 ccttatgatg ttcctgatta cgctggaagt ggaatggggc aacccgggaa cggcagcgcc
      181 ttcttgctgg cacccaatag aagccatgcg ccggaccacg acgtcacgca gcaaagggac
      241 gaggtgtggg tggtgggcat gggcatcgtc atgtctctca tcgtcctggc catcgtgttt
      301 ggcaatgtgc tggtcatcac agccattgcc aagttcgagc gtctgcagac ggtcaccaac
      361 tacttcatca cttcactggc ctgtgctgat ctggtcatgg gcctggcagt ggtgcccttt
      421 ggggccgccc atattcttat gaaaatgtgg acttttggca acttctggtg cgagttttgg
      481 acttccattg atgtgctgtg cgtcacggcc agcattgaga ccctgtgcgt gatcgcagtg
      541 gatcgctact ttgccattac ttcacctttc aagtaccaga gcctgctgac caagaataag
      601 gcccgggtga tcattctgat ggtgtggatt gtgtcaggcc ttacctcctt cttgcccatt
      661 cagatgcact ggtaccgggc cacccaccag gaagccatca actgctatgc caatgagacc
      721 tgctgtgact tcttcacgaa ccaagcctat gccattgcct cttccatcgt gtccttctac
      781 gttcccctgg tgatcatggt cttcgtctac tccagggtct ttcaggaggc caaaaggcag
      841 ctccagaaga ttgacaaatc tgagggccgc ttccatgtcc agaaccttag ccaggtggag
      901 caggatgggc ggacggggca tggactccgc agatcttcca agttctgctt gaaggagcac
      961 aaagccctca agacgttagg catcatcatg ggcactttca ccctctgctg gctgcccttc
     1021 ttcatcgtta acattgtgca tgtgatccag gataacctca tccgtaagga agtttacatc
     1081 ctcctaaatt g
//
