LOCUS       sequence-70573-e         308 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MBP myocilin olfactomedin domain fusion protein with a TEV
            protease recognition sequence and the mutation I499F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Burns JN, Turnage KC, Walker CA, Lieberman RL
  TITLE     The stability of myocilin olfactomedin domain variants provides new 
            insight into glaucoma as a protein misfolding disorder.
  JOURNAL   Biochemistry. 2011 Jul 5;50(26):5824-33. doi: 10.1021/bi200231x. 
            Epub 2011 Jun 9.
  PUBMED    21612213
REFERENCE   2  (bases 1 to 308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi200231x";
            journalName: "Biochemistry"; date: "2011-07-5- 5"; volume: "50"; 
            issue: "26"; pages: "5824-33"
COMMENT     Sequence Label: pmalc4x_I499F_TEV
FEATURES             Location/Qualifiers
     source          1..308
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(78..164)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
       61 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
      121 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct ggcagttccc
      181 tactctcgca tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt
      241 cggcatgggg tcaggtggga ccaccgcgct actgccgcca ggcaaattct gttttatcag
      301 accgcttc
//
