LOCUS       sequence-70608-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rtTA under the control of GAL1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pCH043
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(45..431,432..1052))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MPRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     960..979
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gcggccctat ttgtacagaa ttcttattac gatcctcgcg ccccctaccc accgtactcg
       61 tcaattccaa gggcatcggt aaacatctgc tcaaactcga agtcggccat atccagagcg
      121 ccgtaggggg cggagtcgtg gggggtaaat cccggacccg gggaatcccc gtcccccaac
      181 atgtccagat cgaaatcgtc tagcgcgtcg gcatgcgcca tcgccacgtc ctcgccgtct
      241 aagtggagct cgtcccccag gctgacatcg gtcggggggg ccgtcgacag tctgcgcgtg
      301 tgtcccgcgg ggagaaagga caggcgcgga gccgccagcc ccgcctcttc gggggcgtcg
      361 tcgtccggga gatcgagcag gccctcgatg gtagacccgt aattgttttt cgtacgcgcg
      421 cggctgtacg cggacccact ttcacattta agttgttttt ctaatccgca tatgatcaat
      481 tcaaggccga ataagaaggc tggctctgca ccttggcgat caaataattc gatagcttgt
      541 cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga
      601 tgctcttgtt cttccaatac gcaacctaaa gtaaagtgcc ccacagcgct gagtgcatat
      661 aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt
      721 tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt
      781 agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct
      841 tctaaagggc aaaagtgagt atggtgccta tctaacatct caatgggtaa ggcgtcgagc
      901 aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg
      961 gcgagtttac gggttgttaa accttcgatt ccgaccccat taagcagctc taatgcgctg
     1021 ttaatcactt tacttttatc taatctaggc atggatccta tagttt
//
