LOCUS       sequence-70621-e        1056 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  rtTA positive feedback loop with three tet operators in the GAL1 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1056)
  AUTHORS   Hsu C, Scherrer S, Buetti-Dinh A, Ratna P, Pizzolato J, Jaquet V, 
            Becskei A
  TITLE     Stochastic signalling rewires the interaction map of a multiple 
            feedback network during yeast evolution.
  JOURNAL   Nat Commun. 2012 Feb 21;3:682. doi: 10.1038/ncomms1687.
  PUBMED    22353713
REFERENCE   2  (bases 1 to 1056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-02-21"; volume: "3:682. doi"; pages: " 
            10.1038/ncomms1687"
COMMENT     Sequence Label: pR184
FEATURES             Location/Qualifiers
     source          1..1056
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    1011..1029
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1032..1050
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggtacc
       61 atcatagaga aatcatatga aaaatcaaat acaactctgg gcttggagga agtcattata
      121 agatccggtt acatgggtgt ttgtattgat aacattccct cccaatatag ctcttacaat
      181 aatatgacta cattctccaa ttcaatttgc tatgcaagaa agaatttaag ctcggttccc
      241 ttatacagag acttggaaat tcaactctca aatattgcat cttccagctc caaaacccaa
      301 tcaagcgttg tcttgaacat tttgaagtta gcccaattaa cttcggttaa tgttatacat
      361 ccatatgtct tgatggcaac tgtaatccta acaatcctaa tgtttctatt tattctgtat
      421 gtaactgtcc ctaagttacc attcaaacta gcggttaata aatttctact gttgctaagt
      481 tcaactatag ttttgacatg gggtattggt gcgatgtgga ctcatgtagg gataaatgca
      541 agttacagat tggttccatc atcaagtatg aatataatca ctgtcaagaa gggcaagaag
      601 gcagcagtaa tggcttggtt tagctttgca ttcctccttt tagacagtgt ggttttatgg
      661 ttaatatttt taagggatag gaaaagcttg aaagatgaaa ttgataatgt tccatgtgcc
      721 caaaataggt acaataacta ctcttcggat tcatctacat tgcattccaa agtttagctc
      781 gagggtcaga ttcattatct acaaagactg taaaaagaaa gtggaatatt cattcatatc
      841 atattttttc tattaactgc ctggtttctt ttaaattttt tattggttgt cgacttgaac
      901 ggagtgacaa tatatatata tatatattta ataatgacat cattatctgt aaatctgatt
      961 cttaatgcta ttctagttat gtaagagtgg tcccctagga tattgaagta tccctatcag
     1021 tgatagagaa gtccctatca gtgatagaga atccct
//
