LOCUS       sequence-70697-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSABAD219
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             585..602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(642..659)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      812..898
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg gctagaaata
       61 attttgttta actttaagaa ggagatatac atatgaatgg aaatatttat acacaacctg
      121 tagcacaatg gcacgatcgc ggagaacaag aggtgtttga gtattgtttg gaagatggtt
      181 cattgattcg ggcaacaaaa gaccataagt ttatgactgt tgatggtcaa atgttgccaa
      241 ttgatgaaat atttgaacgt gaattggatt tgatgcgggt tgataatttg ccgaatatca
      301 aaatagccac acgtaaatat ttaggcaaac aaaatgtcta tgacattgga gttgagcgcg
      361 accataattt tgcactcaaa aatggcttca tagcttctaa ttgtttcaat ggtacccagt
      421 acaaacttat cctgaacggt aaaaccctga aaggtgaaac caccaccgaa gctgttgacg
      481 ctgctaccgc ggaaaaagtt ttcaaacagt acgctaacga caacggtgtt gacggtgaat
      541 ggacctacga cgacgctacc aaaacctaca ccgtaacgga attccatcat catcatcatc
      601 attaagcttg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
      661 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
      721 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
      781 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      841 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      901 gccgggagcg gatttgaacg ttgcga
//
