LOCUS       sequence-70746-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription of a mRNA encoding humanized Cas9 nuclease, 
            with 3' UTRs suitable for germline expression in C. elegans, for 
            doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Chiu H, Schwartz HT, Antoshechkin I, Sternberg PW
  TITLE     Transgene-Free Genome Editing in Caenorhabditis elegans Using 
            CRISPR-Cas.
  JOURNAL   Genetics. 2013 Aug 26.
  PUBMED    23979577
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 Aug 26."
COMMENT     Sequence Label: SP6-hCas9-Ce-mRNA
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        42..60
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     42..59
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aaccctcact aaagggaaca aaagctggag ctccaccgcg gatttaggtg acactataga
       61 atacacggaa ttctagatga tccccgcgta ccgagctcag aaaaaatgga caagaagtac
      121 tccattgggc tcgatatcgg cacaaacagc gtcggctggg ccgtcattac ggacgagtac
      181 aaggtgccga gcaaaaaatt caaagttctg ggcaataccg atcgccacag cataaagaag
      241 aacctcattg gcgccctcct gttcgactcc ggggagacgg ccgaagccac gcggctcaaa
      301 agaacagcac ggcgcagata tacccgcaga aagaatcgga tctgctacct gcaggagatc
      361 tttagtaatg agatggctaa ggtggatgac tctttcttcc ataggctgga ggagtccttt
      421 ttggtggagg aggataaaaa gcacgagcgc cacccaatct ttggcaatat cgtggacgag
      481 gtggcgtacc atgaaaagta cccaaccata tatcatctga ggaagaagct tgtagacagt
      541 actgataagg ctgacttgcg gttgatctat ctcgcgctgg cgcatatgat caaatttcgg
      601 ggacacttcc tcatcgaggg ggacctgaac ccagacaaca gcgatgtcga caaactcttt
      661 atccaactgg ttcagactta caatcagctt ttcgaagaga acccgatcaa cgcatccgga
      721 gttgacgcca aagcaatcct gagcgctagg ctgtccaaat cccggcggct cgaaaacctc
//
