LOCUS       sequence-70809-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Aranko AS, Oeemig JS, Kajander T, Iwai H
  TITLE     Intermolecular domain swapping induces intein-mediated protein 
            alternative splicing.
  JOURNAL   Nat Chem Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320.
  PUBMED    23974115
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Aug 25. doi: 10.1038/nchembio.1320."
COMMENT     Sequence Label: pSABAD518
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             105..122
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             132..149
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gacgcttttt atcgcaactc tctactgttt ctccataccc gtttttttgg gctagaaata
       61 attttgttta actttaagaa ggagatatac ccatgggcag cagccatcat catcatcatc
      121 acagcagcgg cctggtgccg cgcggcagcc taggacagta caaacttatc ctgaacggta
      181 aaaccctgaa aggtgaaacc accaccgaag ctgttgacgc tgctaccgcg gaaaaagttt
      241 tcaaacagta cgctaacgac aacggtgttg acggtgaatg gacctacgac gacgctacca
      301 aaacctacac cgtaacggaa ggatccaagt gcgtatcagg tgaaacacca gtttaccttg
      361 cggatggcaa gacaataaaa ataaaggatc tatacagttc tgagagaaaa aaagaagata
      421 acattgttga ggctggttcg ggagaagaga taatacatct aaaagatccc attcaaatat
      481 attcttatgt ggacgggacc atagtcagga gcagatcaag acttctatac aagggcaaga
      541 gctcttatct tgtgaggata gaaactattg gcggaagatc ggtaagcgtt acaccagttc
      601 acaaactctt tgtccttacg gaaaagggta tcgaagaggt tatggcctcc aacctaaagg
      661 taggcgacat gattgctgct gtagcagaaa gcgaatctga agcaagagac tgcggaatga
      721 gcgaggaatg cgtgatggaa gcagaagttt atacgtcact tgaagcgaca ttcgatagag
      781 taaagtctat agcgtacgag aagggtgatt ttgatgtata cgatctttcc gtaccagaat
      841 acggcaggaa ctttataggc ggagagggac ttctcgtact tcacgctgca gttat
//
