LOCUS       sequence-70811-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_ACTG
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..26
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10..26
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgacgttg taaaacgacg gccagtctta agcgtctcat ggcctgaccc cggaccaagt
       61 ggtggctatc gccagcaaca ttggcggcaa gcaagcgctc gaaacggtgc agcggctgtt
      121 gccggtgctg tgccaggacc atggcctgac tccggaccaa gtggtggcta tcgccagcca
      181 cgatggcggc aagcaagcgc tcgaaacggt gcagcggctg ttgccggtgc tgtgccagga
      241 ccatggcctg accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc
      301 gctcgaaacg gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga
      361 ccaagtggtg gctatcgcca gcaacaatgg cggcaagcaa gcgctcgaaa cggtgcagcg
      421 gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg gaccaagtgg tggctatcga
      481 gacgtctaga ccagccagga cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg
      541 tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc ctgttcggtt
      601 cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa
      661 cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg
      721 gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg
      781 atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac
      841 attatga
//
