LOCUS       sequence-70876-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion 
            protein for periplasmic translocation via PelB signal sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Dammeyer T, Timmis KN, Tinnefeld P
  TITLE     Broad host range vectors for expression of proteins with (Twin-) 
            Strep-tag, His-tag and engineered, export optimized yellow 
            fluorescent protein.
  JOURNAL   Microb Cell Fact. 2013 May 20;12(1):49.
  PUBMED    23687945
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-05-20"; volume: "12(1)"; pages: "49"
COMMENT     Sequence Label: pTDpelB-C_sfYFPTwinStrep
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(342..347,348..364,365..371)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    379..395
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     453..518
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
ORIGIN
        1 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      121 attaatgtaa gttagcgcga attgatctgg tttgacagct tatcatcgac tgcacggtgc
      181 accaatgctt ctggcgtcag gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa
      241 atcactgcat aattcgtgtc gctcaaggcg cactcccgtt ctggataatg ttttttgcgc
      301 cgacatcata acggttctgg caaatattct gaaatgagct gttgacaatt aatcatccgg
      361 ctcgtataat gtgtggaatt gtgagcggat aacaatttca caccctaggc cgcggccgcg
      421 cgaattgtta actttaagaa ggagatatac atatgaagta cctgctgccg accgccgccg
      481 ccggcctgct gctgctggcc gcccagccgg ccatggccga tccgaattcg gtgtcgaagg
      541 gcgaagaact gttcaccggc gtggtgccga tcctggtgga actggacggc gacgtgaacg
      601 gccacaagtt ccgcgtgtcg ggcgaaggcg aaggcgacgc caccaacggc aagctgaccc
      661 tgaagttcat ctgcaccacc ggcaagctgc cggtgccgtg gccgaccctg gtgaccaccc
      721 tgggctacgg cctgcagtgc ttcgcccgct acccggacca catgaagcag cacgacttct
      781 tcaagtcggc catgccggaa ggctacgtgc aggaacgcac catctcgttc aaggacgacg
      841 gcacctacaa gacccgcgcc gaagtgaagt tcgaa
//
