LOCUS       sequence-70933-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Ma AC, Lee HB, Clark KJ, Ekker SC
  TITLE     High Efficiency In Vivo Genome Engineering with a Simplified 15-RVD 
            GoldyTALEN Design.
  JOURNAL   PLoS One. 2013 May 29;8(5):e65259. doi: 
            10.1371/journal.pone.0065259. Print 2013.
  PUBMED    23734242
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-29"; volume: "8(5):e65259. doi"; pages: " 
            10.1371/journal.pone.0065259. Print 2013"
COMMENT     Sequence Label: pFUSB4_GCCC
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..26
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     18..35
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     19..35
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gcgtctcatg gcctgacccc
       61 ggaccaagtg gtggctatcg ccagcaacaa tggcggcaag caagcgctcg aaacggtgca
      121 gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag tggtggctat
      181 cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
      241 gtgccaggac catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg
      301 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
      361 gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac
      421 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
      481 ggctatcgag acgtctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
      541 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
      601 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
      661 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
      721 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
      781 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
      841 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
      901 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gta
//
