LOCUS       sequence-71031-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP96 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC153-dual-dCas9VP96-sgExpression
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        115..363
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     115..135
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     286..305
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        382..457
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        554..839
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
       61 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtgagggc
      121 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
      181 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
      241 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
      301 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
      361 accgggtctt cgagaagacc tgttttagag ctagaaatag caagttaaaa taaggctagt
      421 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag agctagaaat
      481 agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc agacaaatgg
      541 ctctagaggt acccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      601 acccccgccc attgacgtca atagtaacgc caatagggac tttccattga cgtcaatggg
      661 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      721 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc cagtacatga
      781 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
//
