LOCUS       sequence-71048-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP64 on pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC91-pmax-dCas9VP64
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..66
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             70..90
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tacaaaaaag caggctccga attcaccggt gccaccatgt acccatacga tgttccagat
       61 tacgcttcgc cgaagaaaaa gcgcaaggtc gaagcgtccg acaagaagta cagcatcggc
      121 ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc
      181 agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa gaacctgatc
      241 ggagccctgc tgttcgacag cggcgaaaca gccgaggcca cccggctgaa gagaaccgcc
      301 agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat cttcagcaac
      361 gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt cctggtggaa
      421 gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga ggtggcctac
      481 cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag caccgacaag
      541 gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg gggccacttc
      601 ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt catccagctg
      661 gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg cgtggacgcc
      721 aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct gatcgcccag
      781 ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag cctgggcctg
      841 acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca gc
//
