LOCUS       sequence-71055-e        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to 
            iRFP) under the CaM Kinase IIa promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(48..974)
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     regulatory      971..980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggctgcctgg cgacggatcc ttcagccata ctagtggcgg ccgctggcac ggctccggcc
       61 tcggcttcgt cttcgacgag agtctcgacc tcgatctccg ttccgccgat gttcagtttg
      121 gtggttttgc ggatatctcc gtgaatcaat atgtgctcgt ggatcaggac gcgcaggtag
      181 tgtcccaaca acccccaaca atttttactc atcagatcaa taatcgtgtg acctacggtg
      241 gagccataga cgctcaggac gccaaaacct tcgggcccca aaatgaagag aattgggaac
      301 ataccccagc tcacgaaaaa cagccatgcc atgccggtca cgacctggcg gcaccgaccc
      361 tttggcacag tatgataacc ctcgatatat gctttggcgg cgtgaaaaaa tgtgttcgcg
      421 ccatagcaca atccaagaca aaagaagatg actttaacat agccggttgc catggcgctg
      481 gtagcccccc acacgatagt cccgatgtct gagacaagga gtcccatggt tctcctgctg
      541 tagtcgttgc tcaggccggt gaggttgctc aggcggataa ggatgacagg acaagtgagc
      601 agccactctg catagcgcag ccactgcacc cggtgtcctg tggcaaggta gagcatagag
      661 ggattcttaa actcaaaaaa gaactcgaga atcaccttaa ccatttcaat ggcgcacaca
      721 tagatctcct cccagccgca tgtagatttc caggtttggt aggcatagaa catcagcagc
      781 aaaatgctga atcctgctgc aagccactgc aggacatttg acgcggtctg agcgccgttc
      841 gtgccgcgag attcaatcca tccggcacag taacattgat cctcagggac caggacggac
      901 ccgttcacca ccacaggatt agtaacgaac aaaagttcgc gtccgacggc agacaaagcg
      961 ccgccatagt ccatggtggc tccggagtcg actctagagg atccggtacc gctctagagc
     1021 tgcccccaga actaggggcc actcgcctgc ccgtgctcct gagtgc
//
