LOCUS       sequence-71072-e         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not 
            for expression. It has to be transferred to a gateway destination 
            vector for expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC1-pCR8-dCas9VP48
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(133..243)
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             complement(262..282)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgatgagca attatttttt ataatgccaa ctttgtacaa gaaagctggg tcgaattcgc
      121 cctttacccg gggagcatgt caaggtcaaa atcgtcaaga gcgtcagcag gcagcatatc
      181 aaggtcaaag tcgtcaaggg catcggctgg gagcatgtct aagtcaaaat cgtcaagggc
      241 gtcggccggc ccgctggcct ccacctttct cttcttcttg gggctgtcgc ctcccagctg
      301 agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc
      361 cagcacctct ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa
      421 ggcggcaggg gctcccagat tggtcagggt aaacaggtgg atgatattct cggcctgctc
      481 tctgatgggc ttatcccggt gcttgttgta ggcggacagc actttgtcca gattagcgtc
      541 ggccaggatc actctcttgg agaactcgct gatctgctcg atgatctcgt ccaggtagtg
      601 cttgtgctgt tccacaaaca gctgtttctg ctcattatcc tcgggggagc ccttcagctt
      661 ctcatagtgg ctggccaggt acaggaagtt cacatatttg gagggcaggg ccagttcgtt
      721 tcccttctgc agttcgccgg cagaggccag cattctcttc cggccgtttt ccagctcgaa
      781 cagggagtac ttaggcagct tgatgatcag gtcctttttc acttctttgt agcccttggc
      841 ttccagaaag tcgatgggat tcttctcgaa gctgcttctt tccatgatgg tgatccccag
      901 cagctctttc acactcttca gtt
//
