LOCUS       sequence-71081-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual expression construct expressing both dCas9VP64 and sgRNA from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC152-dual-dCas9VP64-sgExpression
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..160)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(194..214)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aatcgatata taacatatcg agatcgaaat cgtccagagc atcggagccg agcatgtcca
       61 gatcaaagtc atccaatgcg tcgcttccca acatatccag gtcaaagtca tcgagggcat
      121 cagaacccag catgtcgaga tcgaaatcgt ccagcgcgtc ggcgcgcccg gatccggccg
      181 gcccgctggc ctccaccttt ctcttcttct tggggctgtc gcctcccagc tgagacaggt
      241 cgatccgtgt ctcgtacagg ccggtgatgc tctggtggat cagggtggcg tccagcacct
      301 ctttggtgct ggtgtacctc ttccggtcga tggtggtgtc aaagtacttg aaggcggcag
      361 gggctcccag attggtcagg gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg
      421 gcttatcccg gtgcttgttg taggcggaca gcactttgtc cagattagcg tcggccagga
      481 tcactctctt ggagaactcg ctgatctgct cgatgatctc gtccaggtag tgcttgtgct
      541 gttccacaaa cagctgtttc tgctcattat cctcggggga gcccttcagc ttctcatagt
      601 ggctggccag gtacaggaag ttcacatatt tggagggcag ggccagttcg tttcccttct
      661 gcagttcgcc ggcagaggcc agcattctct tccggccgtt ttccagctcg aacagggagt
      721 acttaggcag cttgatgatc aggtcctttt tcacttcttt gtagcccttg gcttccag
//
