LOCUS       sequence-71083-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP96 on pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC92-pmax-dCas9VP96
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..109
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(137..364)
                     /codon_start=1
                     /product="6 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP96
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDML"
     CDS             complement(398..418)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaccgagga gagggttagg gataggctta ccttcgaacc gcgggccctc tagactcgag
       61 cggccgccac tgtgctggat atcaaccact ttgtacaaga aagctgggtc gaattcttaa
      121 ttaatcaatc gatatacaac atatccaaat cgaaatcatc aagggcatcg cttcccaaca
      181 tatcaaggtc gaaatcatcc agtgcgtctg accctaacat atcgagatcg aaatcgtcca
      241 gagcatcgga gccgagcatg tccagatcaa agtcatccaa tgcgtcgctt cccaacatat
      301 ccaggtcaaa gtcatcgagg gcatcagaac ccagcatgtc gagatcgaaa tcgtccagcg
      361 cgtcggcgcg cccggatccg gccggcccgc tggcctccac ctttctcttc ttcttggggc
      421 tgtcgcctcc cagctgagac aggtcgatcc gtgtctcgta caggccggtg atgctctggt
      481 ggatcagggt ggcgtccagc acctctttgg tgctggtgta cctcttccgg tcgatggtgg
      541 tgtcaaagta cttgaaggcg gcaggggctc ccagattggt cagggtaaac aggtggatga
      601 tattctcggc ctgctctctg atgggcttat cccggtgctt gttgtaggcg gacagcactt
      661 tgtccagatt agcgtcggcc aggatcactc tcttggagaa ctcgctgatc tgctcgatga
      721 tctcgtccag gtagtgcttg tgctgttcca caaacagctg tttctgctca ttatcctcgg
      781 gggagccctt cagcttctca tagtggctgg ccaggtacag gaagttcaca tatttggagg
      841 gcagggccag ttcgtttccc ttctgcagtt cgccggcaga
//
