LOCUS       sequence-71084-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9VP96 on pmax expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC92-pmax-dCas9VP96
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          92..224
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             287..313
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             317..337
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cgtttagtga accgtcagat cactagtagc tttattgcgg tagtttatca cagttaaatt
       61 gctaacgcag tcagtgctcg actgatcaca ggtaagtatc aaggttacaa gacaggttta
      121 aggaggccaa tagaaactgg gcttgtcgag acagagaaga ttcttgcgtt tctgataggc
      181 acctattggt cttactgaca tccactttgc ctttctctcc acagggaaaa aacaattgac
      241 aagtttgtac aaaaaagcag gctccgaatt caccggtgcc accatgtacc catacgatgt
      301 tccagattac gcttcgccga agaaaaagcg caaggtcgaa gcgtccgaca agaagtacag
      361 catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
      421 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
      481 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
      541 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
      601 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
      661 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
      721 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
      781 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
      841 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
      901 ccagctggtg cagacctaca accagctgtt cgaggaaa
//
