LOCUS       sequence-71326-e        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to 
            iRFP) under the CaM Kinase IIa promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Richie CT, Whitaker LR, Whitaker KW, Necarsulmer J, Baldwin HA, 
            Zhang Y, Fortuno L, Hinkle JJ, Koivula P, Henderson MJ, Sun W, Wang 
            K, Smith JC, Pickel J, Ji N, Hope BT, Harvey BK
  TITLE     Near-infrared fluorescent protein iRFP713 as a reporter protein for 
            optogenetic vectors, a transgenic Cre-reporter rat, and other 
            neuronal studies.
  JOURNAL   J Neurosci Methods. 2017 Jun 1;284:1-14. doi: 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2.
  PUBMED    28380331
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci 
            Methods."; date: "2017-06-1"; volume: "284:1-14. doi"; pages: " 
            10.1016/j.jneumeth.2017.03.020. Epub 2017 Apr 2"
COMMENT     Sequence Label: pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      443..452
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gttctcctgc tgtagtcgtt gctcaggccg gtgaggttgc tcaggcggat aaggatgaca
       61 ggacaagtga gcagccactc tgcatagcgc agccactgca cccggtgtcc tgtggcaagg
      121 tagagcatag agggattctt aaactcaaaa aagaactcga gaatcacctt aaccatttca
      181 atggcgcaca catagatctc ctcccagccg catgtagatt tccaggtttg gtaggcatag
      241 aacatcagca gcaaaatgct gaatcctgct gcaagccact gcaggacatt tgacgcggtc
      301 tgagcgccgt tcgtgccgcg agattcaatc catccggcac agtaacattg atcctcaggg
      361 accaggacgg acccgttcac caccacagga ttagtaacga acaaaagttc gcgtccgacg
      421 gcagacaaag cgccgccata gtccatggtg gctccggagt cgactctaga ggatccggta
      481 ccgctctaga gctgccccca gaactagggg ccactcgcct gcccgtgctc ctgagtgcaa
      541 acggagaacc ggcttgactg acgagcttgg ggcttctgag cagggcactg tggctgctca
      601 cagcctcgcg agccttcgtc agcatccagg tccccatggc aaccacctcc gaaacgccct
      661 gttcccgttg ccccggtaac tgcctcccca acacctgcct gcctttcact ttaaaacctg
      721 ctcctctttg ccctggcctt atatacacaa tactgcagga ctgtgtgggc ccaggagcaa
      781 gtggaccctg ttcccccagg gtgatgtact gagggggttg gagaagggga tgcgaacaaa
      841 cttagtccac aagtgatcat cgctcttcct acctccccca cctccaacta tagggccctt
      901 ttaacagacg ggatgggatt aagtggggca aagaggcagt tgctatggta acggctagtg
      961 ggtgccacat tctgggatgg tccttgagaa agacaggatg tagttaccct ggcaacttca
     1021 tctcctctga a
//
