LOCUS       sequence-71345-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human PMCA4 with C-terminal 120aa deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Enyedi A, Verma AK, Filoteo AG, Penniston JT
  TITLE     A highly active 120-kDa truncated mutant of the plasma membrane Ca2+
            pump.
  JOURNAL   J Biol Chem. 1993 May 15;268(14):10621-6.
  PUBMED    8387523
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1993-05-15"; volume: "268(14)"; pages: "10621-6"
COMMENT     Sequence Label: pMM2-hPMCA4bct120
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(188..751)
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
ORIGIN
        1 ttacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
       61 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatctaa agccagcaaa
      121 agtcccatgg tcttataaaa atgcatagct ttaggagggg agcagagaac ttgaaagcat
      181 cttcctgtta gtctttcttc tcgtagactt caaacttata cttgatgcct ttttcctcct
      241 ggacctcaga gaggacgcct gggtattctg ggagaagttt atatttcccc aaatcaattt
      301 ctgggaaaaa cgtgtcactt tcaaattcct gcatgatcct tgtcacaaag agtctgaggt
      361 ggcctggttg attcatggct tcctggtaaa cagaactgcc tccgactatc caaaccatgt
      421 ctactttact tgccaattcc ggttgttcaa taagtcttaa ggcatcatcc aaacttttgg
      481 caagaaaatg agctcctcgt ggtggttctt tgagttctct actgagaact atattaattc
      541 tgtcctttaa aggtcgattc ttctcaggaa tggagaacca ggttttccta cccataatca
      601 ccagattctg tttaccttcc actgaagagg ttgtggtcat tctttggaag tacttgaact
      661 cgttcctgag cggaggccag ggtaggtctc cgttcttgcc aatccccata ttttgggaca
      721 cggcgacgat gcagttcaat ggtcgaacca tgatggcagc ggggataaaa tcctaccagc
      781 cttcacgcta ggattgccgt caagtttggc gcgaaatcgc agccctgagc tgtc
//
