LOCUS       sequence-71440-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G1-Km
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     201..218
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(290..479)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(509..538)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(555..574)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(556..574)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
       61 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      121 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      181 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      241 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggatc ttcgagcgtc
      301 ccaaaacctt ctcaagcaag gttttcagta taatgttaca tgcgtacacg cgtctgtaca
      361 gaaaaaaaag aaaaatttga aatataaata acgttcttaa tactaacata actataaaaa
      421 aataaatagg gacctagact tcaggttgtc taactccttc cttttcggtt agagcggatc
      481 ttagctagcc gcggtaccaa gcttactcga ggtcttcttc ggaaatcaac ttctgttcca
      541 tgtcgacgcc cgggccctat agtgagtcgt attacggatc cttgttttat atttgttgta
      601 aaaagtagat aattacttcc ttgatgatct gtaaaaaaga gaaaaagaaa gcatctaaga
      661 acttgaaaaa ctacgaatta gaaaagacca aatatgtatt tcttgcattg accaatttat
      721 gcaagtttat atatatgtaa atgtaagttt cacgaggttc tactaaacta aaccaccccc
      781 ttggttagaa gaaaagagtg tgtgagaaca ggctgttgtt gtcacacgat tcggacaatt
//
