LOCUS       sequence-71452-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid with EGFP expressed from CMV wild-type 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Ferreira JP, Peacock RW, Lawhorn IE, Wang CL
  TITLE     Modulating ectopic gene expression levels by using retroviral 
            vectors equipped with synthetic promoters.
  JOURNAL   Syst Synth Biol. 2011 Dec;5(3-4):131-8. doi: 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20.
  PUBMED    23205156
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Syst Synth 
            Biol."; date: "2011-12"; volume: "5(3-4):131-8. doi"; pages: " 
            10.1007/s11693-011-9089-0. Epub 2011 Nov 20"
COMMENT     Sequence Label: pCOCK CMVwt
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          61..290
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     273..292
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      327..336
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(333..335,336..338,339..851)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(378..399)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(639..658)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cctttagtga gggttaatgt gctggaatta attcgctgtc tgcgagggcc agctgttggg
       61 gtgagtactc cctctcaaaa gcgggcatga cttctgcgct aagattgtca gtttccaaaa
      121 acgaggagga tttgatattc acctggcccg cggtgatgcc tttgagggtg gccgcgtcca
      181 tctggtcaga aaagacaatc tttttgttgt caagcttgag gtgtggcagg cttgagatct
      241 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
      301 ccaggtccaa ctgcaggtcg agcatcgcca ccatggtgag caagggcgag gagctgttca
      361 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      421 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      481 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      541 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      601 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      661 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      721 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      781 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      841 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      901 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
      961 aagaccccaa cgagaagcgc gatcacatgg tcctgctg
//
