LOCUS       sequence-71581-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E-cadherin EC12 WT with C9A mutation in E.Coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Ritterson RS, Kuchenbecker KM, Michalik M, Kortemme T
  TITLE     Design of a Photoswitchable Cadherin.
  JOURNAL   J Am Chem Soc. 2013 Aug 7.
  PUBMED    23923816
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2013 Aug 7."
COMMENT     Sequence Label: pET22b-EC12WTC9A
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    64..88
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             103..125
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             136..153
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             838..855
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(908..926)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat
       61 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
      121 ggagatatac atatgcacca ccaccaccac cacgaaaacc tgtacttcca ggactgggtc
      181 atccctccca tcagcgcccc cgaaaatgaa aagggcgaat ttccaaagaa cctggttcag
      241 atcaaatcca acagggacaa agaaacaaag gttttctaca gcatcaccgg ccaaggagct
      301 gacaaacccc ccgttggcgt tttcatcatt gagagggaga caggctggct gaaagtgaca
      361 cagcctctgg atagagaagc cattgccaag tacatcctct attctcatgc cgtgtcgtca
      421 aatggggaag cggtggagga tcccatggag atagtgatca cagtgacaga tcagaatgac
      481 aacaggccag agtttaccca gccggtcttt gagggattcg ttgcagaagg cgctgttcca
      541 ggaacctccg tgatgaaggt ctcagccacc gatgcagacg atgacgtcaa cacctacaac
      601 gctgccatcg cctacaccat cgtcagccag gatcctgagc tgcctcacaa aaacatgttc
      661 actgtcaata gggacaccgg ggtcatcagt gtgctcacct ctgggctgga ccgagagagt
      721 taccctacat acactctggt ggttcaggct gctgaccttc aaggtgaagg cttgagcaca
      781 acagccaagg ctgtgatcac tgtcaaggat attaatgaca acgctccttg actcgagcac
      841 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      901 gctgccaccg ctgagcaata actagcataa ccccttggg
//
