LOCUS       sequence-71590-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses E-cadherin EC12 WT with C9A mutation in E.Coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Ritterson RS, Kuchenbecker KM, Michalik M, Kortemme T
  TITLE     Design of a Photoswitchable Cadherin.
  JOURNAL   J Am Chem Soc. 2013 Aug 7.
  PUBMED    23923816
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2013 Aug 7."
COMMENT     Sequence Label: pET22b-EC12WTC9A
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(719..736)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             747..769
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    784..808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(808..827)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(809..827)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tcaaggagcg ttgtcattaa
       61 tatccttgac agtgatcaca gccttggctg ttgtgctcaa gccttcacct tgaaggtcag
      121 cagcctgaac caccagagtg tatgtagggt aactctctcg gtccagccca gaggtgagca
      181 cactgatgac cccggtgtcc ctattgacag tgaacatgtt tttgtgaggc agctcaggat
      241 cctggctgac gatggtgtag gcgatggcag cgttgtaggt gttgacgtca tcgtctgcat
      301 cggtggctga gaccttcatc acggaggttc ctggaacagc gccttctgca acgaatccct
      361 caaagaccgg ctgggtaaac tctggcctgt tgtcattctg atctgtcact gtgatcacta
      421 tctccatggg atcctccacc gcttccccat ttgacgacac ggcatgagaa tagaggatgt
      481 acttggcaat ggcttctcta tccagaggct gtgtcacttt cagccagcct gtctccctct
      541 caatgatgaa aacgccaacg gggggtttgt cagctccttg gccggtgatg ctgtagaaaa
      601 cctttgtttc tttgtccctg ttggatttga tctgaaccag gttctttgga aattcgccct
      661 tttcattttc gggggcgctg atgggaggga tgacccagtc ctggaagtac aggttttcgt
      721 ggtggtggtg gtggtgcata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag
      781 aggggaattg ttatccgctc acaattcccc tatagtgagt cgtattaatt tcgcgggatc
      841 gagatctcga tcctctac
//
