LOCUS       sequence-71598-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Sumo TEV expression vector with BioBr...
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      61..88
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        114..132
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             149..171
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             190..207
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             217..510
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             517..537
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     terminator      579..625
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tctgttgttt gtcggtgagt ccttccagtc tgggcgaaag cgcggtcgat caaattaagc
       61 agaaggccat cctgacggat ggcctttttg cgtttctatc gatcccgcga aattaatacg
      121 actcactata ggcccctcta gaaataattt tgtttaactt taagaaggag atataccatg
      181 ggttcttctc accatcacca tcaccatatg gctagcatgt cggactcaga agtcaatcaa
      241 gaagctaagc cagaggtcaa gccagaagtc aagcctgaga ctcacatcaa tttaaaggtg
      301 tccgatggat cttcagagat cttcttcaag atcaaaaaga ccactccttt aagaaggctg
      361 atggaagcgt tcgctaaaag acagggtaag gaaatggact ccttaagatt cttgtacgac
      421 ggtattagaa ttcaagctga tcagacccct gaagatttgg acatggagga taacgatatt
      481 attgaggctc acagagaaca gattggtggg atcgaggaaa acctgtactt ccaatccaat
      541 attggaagtg gataactggc tgctgccacc gctgagcata gcataacccc ttggggcctc
      601 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatcaagtc ccgaagtagc
      661 tgtcagactg ctggcgaccg ccgcaactag tggcgtcatg cgtgccgtat ggtctttcgc
      721 ctgtagacgc cctacaagcc gtactaaagt ggaaagaccc gcaaaaggta tcgcgatcgc
      781 cctgcaggga actatatccg actagtagcc cgcctaatga gcgggctttt ttttaattcc
      841 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      901 tgataaatgc ttcgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      961 cccttttt
//
