LOCUS       sequence-71599-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G1-Km
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      31..196
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(86..110)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(219..252)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(315..512)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggtagatacg ttgttgacac ttctaaataa gcgaatttct tatgatttat gatttttatt
       61 attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta
      121 aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta
      181 tagcatgagg tcgctcctag tggatctgat atcacctaat aacttcgtat agcatacatt
      241 atacgaagtt atattaaggg ttctcgagag ctcgttttcg acactggatg gcggcgttag
      301 tatcgaatcg acagcagtat agcgaccagc attcacatac gattgacgca tgatattact
      361 ttctgcgcac ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc
      421 acgctaacat ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag
      481 ttcttgaaaa caagaatctt tttattgtca gtactgatta gaaaaactca tcgagcatca
      541 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
      601 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
      661 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
      721 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
      781 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
      841 cactcgcatc aaccaa
//
