LOCUS       sequence-71612-e         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and HXT7 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G4-Km
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(174..197)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta nctaccaacg
       61 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg
      121 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg
      181 tcgtcatcct tgtaatccat cgatactagt tttttgatta aaattaaaaa aactttttgt
      241 ttttgtgttt attctttgtt cttagaaaag acaagttgag cttgtttgtt cttgatgttt
      301 tattatttta caatagctgc aaatgaagaa tagattcgaa cattgtgaag tattggcata
      361 tatcgtctct atttatactt tttttttttc agttctagta tattttgtat tttcctcctt
      421 ttcattcttt cagttgccaa taagttacag gggatctcga aagatggtgg ggatttttcc
      481 ttgaaagacg actttttgcc atctaatttt tccttgttgc ctctgaaaat tatccagcag
      541 aagcaaatgt aaaagatgaa cctcagaaga acacgcaggg gcccgaaatt gttcctacga
      601 gaagtagtgg gtcataaaaa gtttattccc tggaaaaaaa attttgcgtt gcctttctgg
      661 agaatttttt cgaattagcg tgctgccact gcatgcattt ctgagaagtg tgggcattct
      721 tccaccagtt gttcctccta aaaaaaaaaa gatttcctac cccgcacaaa ttcctgcata
      781 cccctcattt ccacggggcc ggccgcacac accatag
//
