LOCUS       sequence-71625-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with G418 
            resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G2-Km
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      34..199
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(89..113)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(222..255)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(318..515)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gttggtagat acgttgttga cacttctaaa taagcgaatt tcttatgatt tatgattttt
       61 attattaaat aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt
      121 ttaaaacgaa aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag
      181 gtatagcatg aggtcgctcc tagtggatct gatatcacct aataacttcg tatagcatac
      241 attatacgaa gttatattaa gggttctcga gagctcgttt tcgacactgg atggcggcgt
      301 tagtatcgaa tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt
      361 actttctgcg cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa
      421 atcacgctaa catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac
      481 aagttcttga aaacaagaat ctttttattg tcagtactga ttagaaaaac tcatcgagca
      541 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
      601 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
      661 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
      721 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
//
