LOCUS       sequence-71641-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) and expresses
            dxs and idi under PxylA promoter (inducible by xylose) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-6xR.ads-PxylA-DI
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gactattcct aataagccga tattagcctc gtatgtttca accatttgtt ggaagcgaga
       61 agaatcataa tggggaaggc catccagcct cgcgtcggat caattcatcg atatctagat
      121 ctttatcgca cactatagct tgatgtattg acccctctct ctgtgagcca atgatgggtt
      181 tcacctttga tcacaagggg cgccttttgt aaatcggcaa ttgttctggc acccagcacg
      241 gtcataatca actttaattc ctcaaggatc agctgaatct cctcaagcag tccttcctca
      301 ccgctgtcag tcagcgcttt taaaaaatgc cctgccattc cggtgcaaga ggcccccagc
      361 gcaattgcct ttgccacgtc aagcgcatct tgcagaccgc cagaggcgat catggtgctt
      421 gcaggaaact cagagcggat ttccgccaaa cttgcagctg tcgaaatgcc ccacgaatta
      481 aaaaaggaga tttgccgctg tcttcggaga ttttcgattt tcgagaaatt tgttcccccg
      541 taaccgccaa tgtcaacagc tgcagcacca gcttcataca gctttcctgc tgatgctttg
      601 ctcataccga agccgacttc tttcacaatg accggtacac tgacccggct gcaaatttgt
      661 tcaatgcgtt tcaatgcgcc gctaaagctt ctgtcccctt caggcatcac aatttcctga
      721 atcacattga ggtggatctg cagtgcgttt gctccaatca tctcaacggc ttcctttgcc
      781 tgagcagccg ttgcctcgct tcccaggttg gcaaaaatca gcccgtt
//
