LOCUS       sequence-71647-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) and expresses
            dxs and idi under PxylA promoter (inducible by xylose) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-6xR.ads-PxylA-DI
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggcatgatt gatgtgttgt ctttttcgtt ctgctcgagt catggttcct tcctcctttg
       61 gatcctcatg atccaattcc tttgtgtgtc tttggtggca tcagtaatct aatacgattt
      121 gccacctgct gttttgtcag tccaatttcc tcaagaagcg ctgttacact tccgtgttca
      181 ataaaccgat caggtatacc cattctgtca atcggagtat gatattcacc ttgatcatga
      241 gcgaattcta aaatcgagct tccgaaaccg ccttctaaga ccgcttcttc aattgttaaa
      301 attggcaagc cttcttttag gatactcttc atcatctttt catcaatcgg cttaataaaa
      361 cgcgcattca caacgcgcac ggaaaggcct tctttctgca gctcttcggc tgcttcaatc
      421 gccatttcga ttgttgtgcc gaatgttaag atgacagcat cgttccctgg acgcagcacc
      481 tcccacgtac cgatcggaat cgttttcaac tgttcatcca tttttacgcc gagtccgttt
      541 ccgcgcggaa aacgcattgc tatcgggcct tcgtcatagc taagtgctgt atgaaccatg
      601 tgctggcctt cattttcgtc tttcggcatc attaagacca tgtttggaat gtggcgcata
      661 aacgcaatat caaacacgcc ttgatgtgtc tctccatcag cgccaacgag tccagcacgg
      721 tcaattccaa taaacacatt agcgttttgg cggcagatgt catgaacaac ttggtcatat
      781 gccctttgca ggaaggttga gtaaatcgcc aaaaacggct tcataccctg cattgccata
      841 gctgcagcca ttgttgcggc atgctgttct gcg
//
