LOCUS       sequence-71649-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ads under Pgrac promoter (inducible by IPTG) and expresses
            dxs and idi under PxylA promoter (inducible by xylose) in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Zhou K, Zou R, Zhang C, Stephanopoulos G, Too HP
  TITLE     Optimization of amorphadiene synthesis in Bacillus subtilis via 
            transcriptional, translational, and media modulation.
  JOURNAL   Biotechnol Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900.
  PUBMED    23483530
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2013 Mar 8. doi: 10.1002/bit.24900."
COMMENT     Sequence Label: pHT-6xR.ads-PxylA-DI
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttttgattca ttttttttac taaagcttga tctgcaattt gaataataac cactcctttg
       61 tttatccacc gaactaagtt ggtgtttttt gaagcttgaa ttagatattt aaaagtatca
      121 tatctaatat tataactaaa ttttctaaaa aaaacattga aataaacatt tattttgtat
      181 atgatgagat aaagttagtt tattggataa acaaactaac tcaattaaga tagttgatgg
      241 ataaacttgt tcacttaaat caaaggggga aatgacaaat ggtccaaact agtgatcttt
      301 tatcaataca ggacccgtcg tttttaaaaa acatgtccat tgatgaatta gagaaattaa
      361 gtgatgaaat ccgtcagttt ttaattacaa gtttatccgc ttccggcggc cacatcggcc
      421 caaacttagg tgtcgtagag cttactgttg ccctgcataa ggaatttaac agcccgaaag
      481 acaaattttt atgggatgta ggccatcagt cgtatgtcca taagctgctg acaggacgcg
      541 gaaaagaatt tgcgacgctt cgccagtaca aagggctttg cggatttcca aagcggagtg
      601 aaagcgagca cgatgtttgg gaaaccgggc acagctcgac ttctctgtca ggcgcgatgg
      661 gaatggcagc tgcccgtgat attaaaggaa cggatgaata tattattccg atcattggtg
      721 acggcgcgct gaccggcggt atggcgctcg aagcccttaa ccacatcggc gacgagaaaa
      781 aagacatgat tgtcatcctt aatgataatg aaatgagtat tgcgccaaac gtcggtgcca
      841 ttcactctat gctcggacgg ct
//
