LOCUS       sequence-71662-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  TITLE     Series-13: Co-transformation vectors for Series-2 transfer vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET His6 Mocr TEV co-transformation cloning ve...
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     125..144
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        125..143
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             232..254
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             274..291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             661..681
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(806..824)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      820..867
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      121 aaattaatac gactcactat agggagacca caacggtttc cctctagtgc cggctccgga
      181 gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa ttttgtttaa
      241 ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca tggttcttct
      301 atgagcaaca tgacatacaa taatgttttc gaccacgcgt acgaaatgct taaggagaac
      361 atccgttatg atgacatacg ggatacagat gaccttcacg acgcaattca tatggccgcc
      421 gataatgccg tccctcacta ttatgccgat attcggtcag ttatggcatc tgaaggtatt
      481 gatctggaat ttgaggatag tggcctcatg ccagatacga aggacgacat cagaattctg
      541 caagcgcgga tctacgaaca acttaccatc gatttatggg aagatgcgga agatctcctg
      601 aatgaatacc tggaagaagt ggaggagtat gaggaagatg aagaggggac ggggatcgag
      661 gaaaacctgt acttccaatc caatattgga agtggataac ggatccgcga tcgcggcgcg
      721 ccacctggtg gccggccggt accacgcgtg cgcgctgatc cggctgctaa caaagcccga
      781 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      841 tctaaacggg tcttgagggg ttttttgctg aaacctcagg catttgagaa gcacacggtc
      901 acactgcttc cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa
      961 cgaccctgcc ctgaaccgac gacaagctga cgaccgggtc tccgcaagtg gcacttttcg
     1021 gggaaatgtg cgcggaaccc ctatttgttt attt
//
