LOCUS       sequence-71717-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG tagged NmCas9 with no NLS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, 
            Thomson JA
  TITLE     Efficient genome engineering in human pluripotent stem cells using 
            Cas9 from Neisseria meningitidis.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 12.
  PUBMED    23940360
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Aug 12."
COMMENT     Sequence Label: pSimpleII-NmCas9-FLAG
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tatcatcatc atccttataa tcacggacag gcgggcgttt tttcagacgg catggtctga
       61 tttctttgcc cagttcgtca atttggtatt tttggaatga aagggcggtt ttgacgccga
      121 taccttccag tattccattt ttgccaattt tatgatcaag atcatgaatg cgtatattga
      181 tattacctgt gcctcgatgg cagctggcaa agtaaccaaa cattctagct ttttttgtta
      241 taacctcgac taaatcatta gggtgtaatg agaatttaaa gttgaaacta tcatcaataa
      301 gttgccaatc ttcttcatct tttccttgta caacagccct atccggcaaa atccctttcg
      361 ctacctgcca actgtaaatc ggtaccagat aatacttgtc gcctttctca aacacatcta
      421 cgcgcaccat ggttgcgttg tcggcaatac cgttatggtt gcgcacccat acgccggttt
      481 tctgtacttg ctctacgcgt acggctttta cctgttgggt gcggttgcct gctttatcgt
      541 atttgtaaaa cggctcggca aaggctttgg caggatcgtc tttatgtgct tccagccgtg
      601 ctttcagtgc ttcgtatagc ttaggttcgc gctcccgatt gaccattttt tccaagtctt
      661 tcagttttaa ctgtgtcagc ggtacgcgca acacgctgac gccttcgtcc agtcgtttgg
      721 cggatttgac ggtctccata tgcccttgcc cgctcatctt ccgattgggc gcgcgtgaaa
      781 caaacagtgg cgtaacgtat tcgtgtacgg cttcggggcg agatgataat ttttcggcaa
      841 gcaacgtgcg cagtttttct agggtatcgg cttcttcgaa ttcgggtttg ccgtccggtt
      901 tgccgaagac gcgaatcatg acttcttgtg cgaaaaattc ccaaggttgt gggaagtgtg
      961 ttttttgatg cagcactt
//
